Anti-Beta1 Adrenergic Receptor Antibodies (IgG) ELISA Kit

Produktnummer ABIN454020

Produktdetails

Target: Anti-Beta1 Adrenergic Receptor Antibodies (IgG)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Verwendungszweck: The anti-beta1-adrenergic-receptor-antibody-EIA is an antibody screening test. The beta1-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-beta1-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-beta1-adrenergic-receptor-antibodies.

Analytische Methode: Quantitative

Anwendungsinformationen

Plattentyp: Pre-coated

Testdurchführung:

Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm

Testpräzision: Intraassay-Precision:9.58% Interassay-Precision:12.02%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Anti-Beta1 Adrenergic Receptor Antibodies (IgG)

Substanzklasse: Antibody

Hintergrund: The beta-1 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The occurrence of auto- antibodies against the beta1-adrenergic-receptor is associated with the existence of ditated cardiomyopathy (DCM). The anti-beta1-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the beta1-adrenergic-receptor in serum and plasma.