GPER ELISA Kit

Produktnummer ABIN421646

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human GPER. Es wurde in 9 Publikationen zitiert.

Kurzübersicht für GPER ELISA Kit (ABIN421646)

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails GPER ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPER in Serum,Plasma,Tissue Homogenate,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of G Protein Coupled Estrogen Receptor 1 (GPER).
No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between G Protein Coupled Estrogen Receptor 1 (GPER) and analogues was observed.

Sensitivität: < 0.125 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Coupled Estrogen Receptor 1 (GPER) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für GPER ELISA Kit (ABIN421646)

Erdem, Şevik Eliçora, Güven, Dinç: "GPER-1 and sex-hormone levels in patients with otosclerosis." in: American journal of otolaryngology, Vol. 41, Issue 3, pp. 102442, (2020) (PubMed).

Barut, Seyithanoglu, Kucukdurmaz, Demir, Olmez, Dogan, Resim: "Relationship between the G protein-coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility." in: Andrologia, pp. e13779, (2020) (PubMed).

Altun, Kurutaş, Şahin, Sınır, Fındıklı: "Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders." in: Psychiatry research, Vol. 257, pp. 67-71, (2018) (PubMed).

Bilal, Kurutas, Orhan: "An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering." in: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, Vol. 275, Issue 2, pp. 469-476, (2018) (PubMed).

Orhan, Kurutaş, Doğaner, Türker, Özcü, Güngör, Çakmak: "Serum levels of GPER-1 in euthymic bipolar patients." in: Neuropsychiatric disease and treatment, Vol. 14, pp. 855-862, (2018) (PubMed).

Sahin, Altun, Kurutaş, Fındıklı: "Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-naïve patients with attention deficit hyperactivity disorder (ADHD)." in: Bosnian journal of basic medical sciences, Vol. 18, Issue 2, pp. 126-131, (2018) (PubMed).

Findikli, Kurutas, Camkurt, Karaaslan, Izci, Fındıklı, Kardaş, Dag, Altun: "Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Naïve Patients with Major Depressive Disorder." in: Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology, Vol. 15, Issue 4, pp. 337-342, (2017) (PubMed).

Fındıklı, Camkurt, Karaaslan, Kurutas, Altun, İzci, Fındıklı, Kardas: "Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder." in: Psychiatry research, Vol. 244, pp. 312-6, (2016) (PubMed).

Kajta, Litwa, Rzemieniec, Wnuk, Lason, Zelek-Molik, Nalepa, Grzegorzewska-Hiczwa, Tokarski, Golas, Guzik, Grochowalski, Szychowski, Wojtowicz: "Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells." in: Molecular and cellular endocrinology, Vol. 392, Issue 1-2, pp. 90-105, (2014) (PubMed).

Details zu GPER

Target: GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

Andere Bezeichnung: GPER

Hintergrund: GPR30, CMKRL2, DRY12, FEG-1, GPCR-Br, LERGU, LyGPR, Membrane estrogen receptor, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1

UniProt: Q99527

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process