RNLS ELISA Kit

Produktnummer ABIN418515

Das Human RNLS ELISA Kit (ABIN418515) ist ein Colorimetric ELISA Kit zur Detektion von Human RNLS. Das Kit wurde in 33 Publikationen zitiert.

Kurzübersicht für RNLS ELISA Kit (ABIN418515)

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails RNLS ELISA Kit

Untere Nachweisgrenze: 3.12 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RNLS in Serum,Plasma,Tissue Homogenate,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Renalase (RNLS).
No significant cross-reactivity or interference between Renalase (RNLS) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Renalase (RNLS) and analogues was observed.

Sensitivität: < 1.31 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renalase (RNLS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renalase (RNLS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für RNLS ELISA Kit (ABIN418515)

You, Jiang, Lin, Li, Wu, Li, Zhou, Ding, Wang, Zhao, Chen, Tang: "The role of urinary renalase on early-stage renal damage in Chinese adults with primary hypertension." in: Experimental biology and medicine (Maywood, N.J.), Vol. 245, Issue 6, pp. 576-582, (2020) (PubMed).

Stojanovic, Mitic, Stojanovic, Petrovic, Ignjatovic, Stefanovic, Cvetkovic, Kocic, Bojanic, Deljanin Ilic: "The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients." in: Current medical research and opinion, Vol. 36, Issue 6, pp. 909-919, (2020) (PubMed).

Musialowska, Zbroch, Koc-Zorawska, Musialowski, Malyszko: "Endocan Concentration in Patients With Primary Hypertension." in: Angiology, Vol. 69, Issue 6, pp. 483-489, (2019) (PubMed).

Lee, Sheu: "Serum Renalase Levels Are Predicted by Brain-Derived Neurotrophic Factor and Associated with Cardiovascular Events and Mortality after Percutaneous Coronary Intervention." in: Journal of clinical medicine, Vol. 7, Issue 11, (2018) (PubMed).

Arya, Emri, Synowsky, Shirran, Barzegar-Befroei, Peto, Botting, Lengyel, Stewart: "Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration." in: Experimental eye research, Vol. 172, pp. 21-29, (2018) (PubMed).

Li, Sheu, Lee, Wang, Fu, Liang, Lee: "Synergistic effect of renalase and chronic kidney disease on endothelin-1 in patients with coronary artery disease ‒ a cross-sectional study." in: Scientific reports, Vol. 8, Issue 1, pp. 7378, (2018) (PubMed).

Lemiesz, Tenderenda-Banasiuk, Sosnowska, Taranta-Janusz, Wasilewska: "Serum Renalase Levels in Adolescents with Primary Hypertension." in: Pediatric cardiology, Vol. 39, Issue 6, pp. 1258-1264, (2018) (PubMed).

Gok Oguz, Akoglu, Ulusal Okyay, Karaveli Gursoy, Yildirim, Merhametsiz, Cimen, Canbakan, Yeter, Ayli: "Increased serum renalase in peritoneal dialysis patients: Is it related to cardiovascular disease risk?" in: Nefrología : publicación oficial de la Sociedad Española Nefrologia, Vol. 37, Issue 2, pp. 189-194, (2017) (PubMed).

Stec: "Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls." in: BMC nephrology, Vol. 18, Issue 1, pp. 118, (2017) (PubMed).

Dziedzic, Powrózek, Orłowska, Koch, Kukula-Koch, Gaweł, Bednarek-Skublewska, Małecka-Massalska, Milanowski, Petkowicz, Solski: "Relationship between microRNA-146a expression and plasma renalase levels in hemodialyzed patients." in: PLoS ONE, Vol. 12, Issue 6, pp. e0179218, (2017) (PubMed).

Dziedzic, Orłowska, Petkowicz, Bednarek-Skublewska, Solski, Goździewska: "Levels of renalase and advanced oxidation protein products with regard to catecholamines in haemodialysed patients." in: Annals of agricultural and environmental medicine : AAEM, Vol. 24, Issue 3, pp. 453-458, (2017) (PubMed).

Wybraniec, Czerwieńska, Lelek, Adamczak, Więcek, Mizia-Stec: "Plasma renalase concentration before and after radiofrequency renal denervation in patients with resistant hypertension." in: Journal of human hypertension, Vol. 30, Issue 6, pp. 410-1, (2016) (PubMed).

Oguz, Gursoy, Yayar, Yildirim, Cimen, Bulut, Eser, Canbakan, Yeter, Ayli: "Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy?" in: Renal failure, Vol. 38, Issue 8, pp. 1180-6, (2016) (PubMed).

Wasilewski, Przybylowski, Wilusz, Sztefko, Janik, Koc-Żórawska, Malyszko: "High-performance Liquid Chromatography Measured Metabolites of Endogenous Catecholamines and Their Relations to Chronic Kidney Disease and High Blood Pressure in Heart Transplant Recipients." in: Transplantation proceedings, Vol. 48, Issue 5, pp. 1751-5, (2016) (PubMed).

Wybraniec, Bożentowicz-Wikarek, Chudek, Mizia-Stec: "Urinary renalase concentration in patients with preserved kidney function undergoing coronary angiography." in: Nephrology (Carlton, Vic.), (2016) (PubMed).

Rama, Llaudó, Fontova, Cerezo, Soto, Javierre, Hueso, Montero, Martínez-Castelao, Torras, Grinyó, Cruzado, Lloberas: "Online Haemodiafiltration Improves Inflammatory State in Dialysis Patients: A Longitudinal Study." in: PLoS ONE, Vol. 11, Issue 10, pp. e0164969, (2016) (PubMed).

Wang, Wang, Chu, Mu, Wang, Liu, Xie, Yang, Dong, Yuan: "Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults." in: Cardiology, Vol. 130, Issue 4, pp. 242-8, (2015) (PubMed).

Malyszko, Koc-Zorawska, Zorawski, Kozminski, Zbroch, Rysz, Banach, Malyszko: "Renalase is removed by kidneys and during dialysis - excess related to CKD complications?" in: Current vascular pharmacology, Vol. 13, Issue 1, pp. 134-40, (2015) (PubMed).

Maciorkowska, Zbroch, Malyszko: "Circulating renalase, catecholamines, and vascular adhesion protein 1 in hypertensive patients." in: Journal of the American Society of Hypertension : JASH, Vol. 9, Issue 11, pp. 855-64, (2015) (PubMed).

Qi, Wang, Zhang, Shao, Chang, Fan, Cao, Mou, Wang, Yan, Desir, Ni: "Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139627, (2015) (PubMed).

Details zu RNLS

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Andere Bezeichnung: RNLS

Hintergrund: MAO-C, C10orf59, FAD-Dependent Amine Oxidase, Monoamine oxidase-C, alpha-NAD(P)H oxidase/anomerase

UniProt: Q5VYX0