CXCL12 ELISA Kit

Produktnummer ABIN414920

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CXCL12. Es wurde in 16 Publikationen zitiert.

Kurzübersicht für CXCL12 ELISA Kit (ABIN414920)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 pg/mL - 200 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails CXCL12 ELISA Kit

Untere Nachweisgrenze: 3.12 pg/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SDF1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1).
No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Sensitivität: < 1.31 pg/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für CXCL12 ELISA Kit (ABIN414920)

Chen, Li, Chen, Han, Li, Guo, Liu, Xiao: "Enhanced recruitment and hematopoietic reconstitution of bone marrow-derived mesenchymal stem cells in bone marrow failure by the SDF-1/CXCR4." in: Journal of tissue engineering and regenerative medicine, Vol. 14, Issue 9, pp. 1250-1260, (2020) (PubMed).

Zhang, Hwang, Oh, Park, Chung, Lee, Baek, Ryoo, Woo: "Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study." in: Biomaterials, Vol. 149, pp. 77-87, (2018) (PubMed).

Wang, Li, Qiu, Hu, Chen, Fu, Zhou, Song: "Low‑intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1‑mediated SDF‑1 expression." in: International journal of molecular medicine, Vol. 42, Issue 1, pp. 322-330, (2018) (PubMed).

Ogłodek, Szota, Just, Szromek, Araszkiewicz: "A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity." in: Pharmacological reports : PR, Vol. 68, Issue 4, pp. 756-63, (2016) (PubMed).

Tsai, Lin, Lin, Hsu, Wang: "High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cells and depressed shedding of vascular endothelial cells undergoing hypoxia." in: European journal of applied physiology, Vol. 116, Issue 11-12, pp. 2375-2388, (2016) (PubMed).

Chen, Lu, Wang, Chen, Lin, Ma, Liu, Zhao, Chen: "Circulating CD133+ CD34+ progenitor cells and plasma stromal-derived factor-1alpha: predictive role in ischemic stroke patients." in: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, Vol. 24, Issue 2, pp. 319-26, (2015) (PubMed).

Og?odek, Szota, Just, Mo?, Araszkiewicz: "The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders." in: Pharmacological reports : PR, Vol. 67, Issue 1, pp. 85-9, (2015) (PubMed).

Meng, Huang, Di, Mu, Wang, Zhao, Zhao, Zhu, Li, Kong, Xing: "Expression of Human Epidermal Growth Factor Receptor-2 in Resected Rectal Cancer." in: Medicine, Vol. 94, Issue 47, pp. e2106, (2015) (PubMed).

Guo, Chai, Chen, Chen, Ge, Li, Li, Li, Cao: "Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 6, pp. 578-85, (2015) (PubMed).

Ogłodek, Szota, Moś, Araszkiewicz, Szromek: "Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder." in: Pharmacological reports : PR, Vol. 67, Issue 6, pp. 1251-8, (2015) (PubMed).

Chiva-Blanch, Condines, Magraner, Roth, Valderas-Martínez, Arranz, Casas, Martínez-Huélamo, Vallverdú-Queralt, Quifer-Rada, Lamuela-Raventos, Estruch: "The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: a randomized clinical trial." in: Atherosclerosis, Vol. 233, Issue 2, pp. 518-24, (2014) (PubMed).

Og?odek, Szota, Just, Mo?, Araszkiewicz: "Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression." in: Pharmacological reports : PR, Vol. 66, Issue 5, pp. 920-6, (2014) (PubMed).

Li, Wang, Zhang, Zhao, Huang, Wu, Li, Li, Liu, Cao, Dai, Fang, Shang, Cao, Zhao, Chen: "Elevated PLGF contributes to small-cell lung cancer brain metastasis." in: Oncogene, Vol. 32, Issue 24, pp. 2952-62, (2013) (PubMed).

Wang, Lee, Lien, Weng: "Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men." in: International journal of cardiology, Vol. 170, Issue 3, pp. 315-23, (2013) (PubMed).

Patschan, Patschan, Henze, Blaschke, Wessels, Müller: "Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis." in: International journal of nephrology, Vol. 2012, pp. 715049, (2012) (PubMed).

Patschan, Patschan, Temme, Korsten, Wessels, Koziolek, Henze, Müller: "Endothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)." in: Critical care, Vol. 15, Issue 2, pp. R94, (2011) (PubMed).

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: SDF1

Hintergrund: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling