Lipocalin 2 ELISA Kit

Produktnummer ABIN411402

Das Maus Lipocalin 2 ELISA Kit (ABIN411402) ist ein Colorimetric ELISA Kit zur Detektion von Maus Lipocalin 2. Das Kit wurde in 4 Publikationen zitiert.

Kurzübersicht für Lipocalin 2 ELISA Kit (ABIN411402)

Target: Lipocalin 2 (LCN2)

Bindungsspezifität: AA 21-200

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine

Produktdetails Lipocalin 2 ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Verwendungszweck: Sandwich Mouse Lipocalin-2/NGAL ELISA Kit to quantitate Mouse Lcn2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: Q21-N200

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.4%, 4.9%, 4.4%
Inter-Assay Precision (CV%): 5.8%, 6.7%, 6.2%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Lipocalin 2 ELISA Kit (ABIN411402)

Liu, Dong, Sun, Liu: "A novel fluid resuscitation protocol: provide more protection on acute kidney injury during septic shock in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 4, pp. 919-26, (2014) (PubMed).

Sun, Meng, Jiang, Liu, Lei, Su, Duan, Wu, Xia, Xia: "Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice." in: PLoS ONE, Vol. 8, Issue 12, pp. e80859, (2013) (PubMed).

Koca, Olguner, Ergür, Altekin, Ta?dö?en, Duru, Girgin, Gündüz, Cilaker M?c?l?, Güzelda?, Akku?: "The effects of dexmedetomidine on secondary acute lung and kidney injuries in the rat model of intra-abdominal sepsis." in: TheScientificWorldJournal, Vol. 2013, pp. 292687, (2013) (PubMed).

Olguner, Koca, Altekin, Ergür, Duru, Girgin, Ta?dö?en, Gündüz, Güzelda?, Akku?, Micili: "Ischemic preconditioning attenuates lipid peroxidation and apoptosis in the cecal ligation and puncture model of sepsis." in: Experimental and therapeutic medicine, Vol. 5, Issue 6, pp. 1581-1588, (2013) (PubMed).

Details zu Lipocalin 2

Target: Lipocalin 2 (LCN2)

Andere Bezeichnung: LCN2

Hintergrund:

Background: Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.

Gene Full Name: lipocalin 2

Gen-ID: 16819

UniProt: P11672

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis