Myeloperoxidase ELISA Kit

Produktnummer ABIN411400

Human Myeloperoxidase ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Myeloperoxidase und es wurde in 7+ Publikationen zitiert.

Kurzübersicht für Myeloperoxidase ELISA Kit (ABIN411400)

Target: Myeloperoxidase (MPO)

Bindungsspezifität: AA 49-745

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine

Produktdetails Myeloperoxidase ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Verwendungszweck: Sandwich Human MPO/Myeloperoxidase ELISA Kit to quantitate Human MPO in cell culture supernatants, cell lysate, serum, plasma (heparin, EDTA) , saliva and urine.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: A49-S745

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.4%, 6.8%, 4.2%
Inter-Assay Precision (CV%): 8.2%, 7.9%, 5.5%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Myeloperoxidase ELISA Kit (ABIN411400)

Fu, Li, Sun, Ma, Yao: "Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways." in: Oncotarget, Vol. 6, Issue 28, pp. 24675-89, (2016) (PubMed).

Liu, Chen, Wu, Chen, Zhang: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." in: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).

Sousa, Morad, Feliciano, Pita, Nady, El-Hennamy, Abdel-Rahman, Cavaco, Pereira, Barreto, Leitão: "The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients." in: AMB Express, Vol. 6, Issue 1, pp. 41, (2016) (PubMed).

Chen, Yang, Liu, Teng, Ye, Xu, Cai, Cheng, Yang, Hu, Wang, Cao: "Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice." in: Journal of ethnopharmacology, Vol. 166, pp. 149-56, (2015) (PubMed).

Wang, Tang, Li, Wang: "Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 121-5, (2015) (PubMed).

Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

Chen, Chen, Qin: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).

Details zu Myeloperoxidase

Target: Myeloperoxidase (MPO)

Andere Bezeichnung: MPO

Hintergrund:

Background: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.

Gene Full Name: myeloperoxidase

Gen-ID: 4353

UniProt: P05164

Pathways: Chromatin Binding