CXCL9 ELISA Kit

Produktnummer ABIN411395

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CXCL9.

Kurzübersicht für CXCL9 ELISA Kit (ABIN411395)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Bindungsspezifität: AA 23-125

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails CXCL9 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CXCL9

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: T23-T125

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: T23-T125

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Plattentyp: Pre-coated

Protokoll: human CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL9 has been precoated onto 96-well plates. Standards(E.coli, T23-T125) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL9 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CXCL9 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 249, Standard deviation: 11.5, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 834, Standard deviation: 35.03, CV(%): 4.2
• Sample 3: n=16, Mean(pg/ml): 1573, Standard deviation: 64.5, CV(%): 4.1,
• Sample 1: n=24, Mean(pg/ml): 231, Standard deviation: 14.1, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 759, Standard deviation: 63.8, CV(%): 8.4
• Sample 3: n=24, Mean(pg/ml): 1628, Standard deviation: 117.2, CV(%): 7.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Andere Bezeichnung: CXCL9

Hintergrund:

Protein Function: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

Background: Chemokine(C-X-C motif) ligand 9(CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon(MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Synonyms: C-X-C motif chemokine 9,Gamma-interferon-induced monokine,Monokine induced by interferon-gamma,HuMIG,MIG,Small-inducible cytokine B9,CXCL9,CMK, MIG, SCYB9,

Full Gene Name: C-X-C motif chemokine 9

Cellular Localisation: Secreted.

Gen-ID: 4283

UniProt: Q07325