LTA ELISA Kit

Produktnummer ABIN411381

Dieses Chemical LTA ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Chemical LTA in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) und Plasma (citrate) zu quantifizieren. Dieses ELISA Kit wurde in 2+ Publikationen zitiert.

Kurzübersicht für LTA ELISA Kit (ABIN411381)

Target: LTA (Lymphotoxin-alpha (LTA))

Bindungsspezifität: AA 35-205

Reaktivität: Chemical

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails LTA ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNF beta

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: L35-L205

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: L35-L205

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the tumor necrosis factor family.

Plattentyp: Pre-coated

Protokoll: human TNF beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNF beta has been precoated onto 96-well plates. Standards(E.coli, L35-L205) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNF beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNF beta amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 415, Standard deviation: 23.24, CV(%): 5.6
• Sample 2: n=16, Mean(pg/ml): 1952, Standard deviation: 68.32, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 2822, Standard deviation: 129.8, CV(%): 4.6,
• Sample 1: n=24, Mean(pg/ml): 660, Standard deviation: 40.92, CV(%): 6.2
• Sample 2: n=24, Mean(pg/ml): 2546, Standard deviation: 122.2, CV(%): 4.8
• Sample 3: n=24, Mean(pg/ml): 3148, Standard deviation: 210.9, CV(%): 6.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für LTA ELISA Kit (ABIN411381)

Wang, Zheng, Meng, Wang, He, Zhang, Liu, Hu, He, Hu, Wang: "In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus." in: Biomaterials, Vol. 64, pp. 78-87, (2015) (PubMed).

Zeng, Huang, Zheng, Guo, Pan: "Effects of reactive nitrogen scavengers on NK-cell-mediated killing of K562 cells." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 101737, (2012) (PubMed).

Details zu LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Andere Bezeichnung: LTA

Substanzklasse: Chemical

Hintergrund:

Protein Function: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.

Background: Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Synonyms: Lymphotoxin-alpha,LT-alpha,TNF-beta,Tumor necrosis factor ligand superfamily member 1,LTA,TNFB, TNFSF1,

Full Gene Name: Lymphotoxin-alpha

Cellular Localisation: Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.

Gen-ID: 4049

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process