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Resistin ELISA Kit

This anti-Resistin antibody (ABIN411379) is a antibody detecting Resistin in ELISA. Suitable for Human.
Produktnummer ABIN411379

Kurzübersicht für Resistin ELISA Kit (ABIN411379)

Target

Alle Resistin (RETN) ELISA Kits anzeigen
Resistin (RETN)

Bindungsspezifität

AA 19-108

Reaktivität

  • 8
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

78-5000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    78 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Resistin

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: E.coli
    Immunogen sequence: K19-P108

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <3pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: K19-P108
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the resistin/FIZZ family.

    Tissue Specificity: Expressed only in fatty tissues.

    Plattentyp

    Pre-coated

    Protokoll

    human Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Resistin has been precoated onto 96-well plates. Standards(E.coli, K19-P108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Resistin amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Resistin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Resistin standard solution and each sample is measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 534, Standard deviation: 28.84, CV(%): 5.4
    • Sample 2: n=16, Mean(pg/ml): 1453, Standard deviation: 88.63, CV(%): 6.1
    • Sample 3: n=16, Mean(pg/ml): 2323, Standard deviation: 109.2, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 554, Standard deviation: 45.98, CV(%): 8.3
    • Sample 2: n=24, Mean(pg/ml): 1532, Standard deviation: 119.5, CV(%): 7.8
    • Sample 3: n=24, Mean(pg/ml): 2486, Standard deviation: 200, CV(%): 8

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Montazerifar, Bolouri, Paghalea, Mahani, Karajibani: "Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease." in: Arquivos brasileiros de cardiologia, Vol. 107, Issue 4, pp. 348-353, (2017) (PubMed).

    Niu, Li, Li, Song, Jin, Liu: "Serum resistin positively correlates with serum lipids, but not with insulin resistance, in first-degree relatives of type-2 diabetes patients: an observational study in China." in: Medicine, Vol. 96, Issue 16, pp. e6622, (2017) (PubMed).

  • Target Alle Resistin (RETN) ELISA Kits anzeigen

    Resistin (RETN)

    Andere Bezeichnung

    RETN

    Hintergrund

    Protein Function: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.

    Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

    Synonyms: Resistin,Adipose tissue-specific secretory factor,ADSF,C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein,Cysteine-rich secreted protein A12-alpha-like 2,Cysteine-rich secreted protein FIZZ3,RETN,FIZZ3, HXCP1, RSTN,UNQ407/PRO1199,

    Full Gene Name: Resistin

    Cellular Localisation: Secreted.

    Gen-ID

    56729

    UniProt

    Q9HD89

    Pathways

    Feeding Behaviour, Smooth Muscle Cell Migration
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