VCAM1 ELISA Kit

Produktnummer ABIN411368

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus VCAM1. Es wurde in 6 Publikationen zitiert.

Kurzübersicht für VCAM1 ELISA Kit (ABIN411368)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Bindungsspezifität: AA 25-698

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails VCAM1 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Mouse VCAM-1 ELISA Kit to quantitate Mouse Vcam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: F25-E698

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.8%, 7.2%, 6.8%
Inter-Assay Precision (CV%): 8.2%, 8.7%, 7.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für VCAM1 ELISA Kit (ABIN411368)

Zhang, Mu, Zhou, Song, Zhang, Wu, Jiang, Wang, Zhang, Li, Shao, Wang, Li, Yang, Wu, Zhang, Tang: "Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction." in: PLoS ONE, Vol. 11, Issue 6, pp. e0158038, (2017) (PubMed).

Xu, Li, Dong, Tan, Wu, Xie, Yang: "Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE-/- Mice through the ROS/MAPK/NF-κB Pathway." in: Nutrients, Vol. 7, Issue 8, pp. 7085-105, (2016) (PubMed).

Barros, Borges, Ferreira, Carmo, Rosado, Fouque, Mafra: "Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?" in: Future microbiology, Vol. 10, Issue 4, pp. 517-26, (2015) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." in: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." in: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).

Asgary, Kelishadi, Rafieian-Kopaei, Najafi, Najafi, Sahebkar: "Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents." in: Pediatric cardiology, Vol. 34, Issue 7, pp. 1729-35, (2013) (PubMed).

Details zu VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Andere Bezeichnung: VCAM1

Hintergrund:

Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.

Gene Full Name: vascular cell adhesion molecule 1

Gen-ID: 22329

UniProt: P29533

Pathways: Carbohydrate Homeostasis