TIMP3 ELISA Kit

Produktnummer ABIN411360

Dieses Human TIMP3 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human TIMP3 zu quantifizieren. Dieses ELISA Kit wurde in 1 Publikation zitiert.

Kurzübersicht für TIMP3 ELISA Kit (ABIN411360)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Bindungsspezifität: AA 24-211

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Produktdetails TIMP3 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TIMP-3

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: C24-P211

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <2pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: C24-P211

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

Plattentyp: Pre-coated

Protokoll: human TIMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-3 has been precoated onto 96-well plates. Standards(NSO, C24-P211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TIMP-3 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1250, Standard deviation: 72.5, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 2687, Standard deviation: 110.2, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 5353, Standard deviation: 192.7, CV(%): 3.6,
• Sample 1: n=24, Mean(pg/ml): 1430, Standard deviation: 95.81, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 2832, Standard deviation: 147.3, CV(%): 5.2
• Sample 3: n=24, Mean(pg/ml): 5657, Standard deviation: 277.2, CV(%): 4.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für TIMP3 ELISA Kit (ABIN411360)

Denkovskij, Bagdonas, Kusleviciute, Mackiewicz, Unguryte, Porvaneckas, Fleury, Venalis, Jorgensen, Bernotiene: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." in: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).

Details zu TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Andere Bezeichnung: TIMP3

Hintergrund:

Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).

Synonyms: Metalloproteinase inhibitor 3,Protein MIG-5,Tissue inhibitor of metalloproteinases 3,TIMP-3,TIMP3,

Full Gene Name: Metalloproteinase inhibitor 3

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gen-ID: 7078

UniProt: P35625