Osteopontin ELISA Kit

Produktnummer ABIN411344

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus Osteopontin. Es sind 2 Publikationen verfügbar.

Kurzübersicht für Osteopontin ELISA Kit (ABIN411344)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Bindungsspezifität: AA 17-294

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Produktdetails Osteopontin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse OPN

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: L17-N294

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: L17-N294

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse OPN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for OPN has been precoated onto 96-well plates. Standards(NSO, L17-N294) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse OPN amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse OPN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse OPN standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1243, Standard deviation: 97, CV(%): 7.8
• Sample 2: n=16, Mean(pg/ml): 3386, Standard deviation: 210, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 5637, Standard deviation: 304.4, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 1448, Standard deviation: 124.6, CV(%): 8.6
• Sample 2: n=24, Mean(pg/ml): 3563, Standard deviation: 295.8, CV(%): 8.3
• Sample 3: n=24, Mean(pg/ml): 6125, Standard deviation: 520.6, CV(%): 8.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Osteopontin ELISA Kit (ABIN411344)

Qin, Yan, Wang, Xu, Wang, Zhu, Shi, Li, Zhang, Chen: "Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway." in: Theranostics, Vol. 8, Issue 4, pp. 921-940, (2018) (PubMed).

Chen, Sun, He, Gong, Xu, Yuan: "Serum OPN expression for identification of gastric cancer and atrophic gastritis and its influencing factors." in: PLoS ONE, Vol. 9, Issue 12, pp. e114005, (2014) (PubMed).

Details zu Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: SPP1

Hintergrund:

Background: Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.

Synonyms: Putative uncharacterized protein ,Spp1 ,

Full Gene Name: secreted phosphoprotein 1

Gen-ID: 20750

UniProt: Q3UBR1

Pathways: Regulation of Cell Size