Osteopontin ELISA Kit

Produktnummer ABIN411343

Produktdetails Osteopontin ELISA Kit

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Bindungsspezifität: AA 17-300

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human OPN

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk, Urine

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: I17-N300

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: I17-N300

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the osteopontin family.

Tissue Specificity: Bone. Found in plasma.

Plattentyp: Pre-coated

Protokoll: human OPN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OPN has been precoated onto 96-well plates. Standards(NSO, I17-N300) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human OPN amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human OPN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin, EDTA), milk or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human OPN standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 2.5, Standard deviation: 0.105, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 4.3, Standard deviation: 0.146, CV(%): 3.4
• Sample 3: n=16, Mean(ng/ml): 6.1, Standard deviation: 0.317, CV(%): 5.2,
• Sample 1: n=24, Mean(ng/ml): 2.6, Standard deviation: 0.161, CV(%): 6.2
• Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.226, CV(%): 5.8
• Sample 3: n=24, Mean(ng/ml): 6.3, Standard deviation: 0.353, CV(%): 5.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für Osteopontin ELISA Kit (ABIN411343)

Qin, Yan, Wang, Xu, Wang, Zhu, Shi, Li, Zhang, Chen: "Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway." in: Theranostics, Vol. 8, Issue 4, pp. 921-940, (2018) (PubMed).

El-Din, Amin, Egiza: "Utility of Tissue Inhibitor Metalloproteinase-1 and Osteopontin as Prospective Biomarkers of Early Cardiovascular Complications in Type 2 Diabetes." in: Open access Macedonian journal of medical sciences, Vol. 6, Issue 2, pp. 314-319, (2018) (PubMed).

Sun, Tu, Chen, Yuan, Liu, Dong, Yuan: "Three-dimensional combined biomarkers assay could improve diagnostic accuracy for gastric cancer." in: Scientific reports, Vol. 7, Issue 1, pp. 11621, (2017) (PubMed).

Chen, Sun, He, Gong, Xu, Yuan: "Serum OPN expression for identification of gastric cancer and atrophic gastritis and its influencing factors." in: PLoS ONE, Vol. 9, Issue 12, pp. e114005, (2014) (PubMed).

Details zu Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: SPP1 (SPP1 Produkte)

Synonyme: BNSP ELISA Kit, BSPI ELISA Kit, ETA-1 ELISA Kit, OPN ELISA Kit, 2AR ELISA Kit, Apl-1 ELISA Kit, Bsp ELISA Kit, Eta ELISA Kit, OP ELISA Kit, Opn ELISA Kit, Opnl ELISA Kit, Ric ELISA Kit, Spp-1 ELISA Kit, OSP ELISA Kit, zgc:111821 ELISA Kit, secreted phosphoprotein 1 ELISA Kit, SPP1 ELISA Kit, Spp1 ELISA Kit, spp1 ELISA Kit

Hintergrund:

Protein Function: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Background: Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.

Synonyms: Osteopontin,Bone sialoprotein 1,Nephropontin,Secreted phosphoprotein 1,SPP-1,Urinary stone protein,Uropontin,SPP1,BNSP, OPN,PSEC0156,

Full Gene Name: Osteopontin

Cellular Localisation: Secreted.

Gen-ID: 6696

UniProt: P10451

Pathways: Regulation of Cell Size