Nerve Growth Factor ELISA Kit

Produktnummer ABIN411336

Das Maus Nerve Growth Factor ELISA Kit (ABIN411336) ist ein Colorimetric ELISA Kit zur Detektion von Maus Nerve Growth Factor. Das Kit wurde in 5 Publikationen zitiert.

Kurzübersicht für Nerve Growth Factor ELISA Kit (ABIN411336)

Target: Nerve Growth Factor (NGF)

Bindungsspezifität: AA 122-241

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails Nerve Growth Factor ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Verwendungszweck: Sandwich Mouse NGF/NGF Beta ELISA Kit to quantitate Mouse Ngf in cell culture supernatants and serum.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: S122-G241

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.5%, 7.4%, 7.7%
Inter-Assay Precision (CV%): 6.5%, 7.4%, 8.3%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Nerve Growth Factor ELISA Kit (ABIN411336)

Wada, Shimizu, Takai, Shimizu, Kanai, Tyagi, Kakizaki, Yoshimura: "Post-injury bladder management strategy influences lower urinary tract dysfunction in the mouse model of spinal cord injury." in: Neurourology and urodynamics, Vol. 36, Issue 5, pp. 1301-1305, (2018) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Fink, Connor, Kelley, Steele, Hollingsworth, Tempero: "Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution." in: PLoS ONE, Vol. 9, Issue 11, pp. e112737, (2014) (PubMed).

Tan, Xie, Liu, Chen, Zheng, Tong, Tian: "Low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) reverses A?(1-42)-mediated memory deficits in rats." in: Experimental gerontology, Vol. 48, Issue 8, pp. 786-94, (2013) (PubMed).

Wang, Zhao, Fan, Gong, Li, Qi, Liu: "Research on the mechanism of Zuogui Pill and Yougui Pill in promoting axonal regeneration in model rats of autoimmune encephalomyelitis." in: Chinese journal of integrative medicine, Vol. 16, Issue 2, pp. 167-72, (2010) (PubMed).

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF

Hintergrund:

Background: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

Gene Full Name: nerve growth factor

Gen-ID: 18049

UniProt: P01139

Pathways: Regulation of Cell Size