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MMP8 ELISA Kit

MMP8 Reaktivität: Human AA 21-467 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Saliva, Serum
Produktnummer ABIN411332
  • Target Alle MMP8 ELISA Kits anzeigen
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    Bindungsspezifität
    AA 21-467
    Reaktivität
    • 7
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156-10000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-8
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Saliva
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: F21-G467
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: F21-G467
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the peptidase M10A family.

    Tissue Specificity: Neutrophils.

    Plattentyp
    Pre-coated
    Protokoll
    human MMP-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, F21-G467) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-8 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-8 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 1275, Standard deviation: 56.1, CV(%): 4.4
    • Sample 2: n=16, Mean(pg/ml): 3563, Standard deviation: 185.3, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 395.7, CV(%): 5.8,
    • Sample 1: n=24, Mean(pg/ml): 1370, Standard deviation: 69.87, CV(%): 5.1
    • Sample 2: n=24, Mean(pg/ml): 3728, Standard deviation: 242.32, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 7142, Standard deviation: 521.4, CV(%): 7.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Kumar, Jain, Chauhan, Bharate, Koli, Kumar: "Influence of different forms and materials (zirconia or titanium) of abutments in periimplant soft-tissue healing using matrix metalloproteinase-8: A randomized pilot study." in: The Journal of prosthetic dentistry, (2017) (PubMed).

    Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

    Akbari, Prabhuji, Karthikeyan, Raghunatha, Narayanan: "Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test." in: Journal of Indian Society of Periodontology, Vol. 19, Issue 5, pp. 525-30, (2015) (PubMed).

  • Target Alle MMP8 ELISA Kits anzeigen
    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
    Andere Bezeichnung
    MMP8 (MMP8 Produkte)
    Synonyme
    MMP8 ELISA Kit, BB138268 ELISA Kit, CLG1 ELISA Kit, HNC ELISA Kit, MMP-8 ELISA Kit, PMNL-CL ELISA Kit, matrix metallopeptidase 8 ELISA Kit, neutrophil collagenase ELISA Kit, MMP8 ELISA Kit, Mmp8 ELISA Kit, LOC100339790 ELISA Kit
    Hintergrund

    Protein Function: Can degrade fibrillar type I, II, and III collagens.

    Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

    Synonyms: Neutrophil collagenase,3.4.24.34,Matrix metalloproteinase-8,MMP-8,PMNL collagenase,PMNL-CL,MMP8,CLG1,

    Full Gene Name: Neutrophil collagenase

    Cellular Localisation: Cytoplasmic granule. Secreted, extracellular space, extracellular matrix . Stored in intracellular granules.
    Gen-ID
    4317
    UniProt
    P22894
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