MMP8 ELISA Kit

Produktnummer ABIN411332

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human MMP8. Es wurde in 3 Publikationen zitiert.

Kurzübersicht für MMP8 ELISA Kit (ABIN411332)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Bindungsspezifität: AA 21-467

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Saliva

Produktdetails MMP8 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Human MMP-8 ELISA Kit to quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: F21-G467

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5.2%, 6.7%, 7.2%
Inter-Assay Precision (CV%): 7.1%, 7.1%, 7.9%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für MMP8 ELISA Kit (ABIN411332)

Kumar, Jain, Chauhan, Bharate, Koli, Kumar: "Influence of different forms and materials (zirconia or titanium) of abutments in periimplant soft-tissue healing using matrix metalloproteinase-8: A randomized pilot study." in: The Journal of prosthetic dentistry, (2017) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Akbari, Prabhuji, Karthikeyan, Raghunatha, Narayanan: "Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test." in: Journal of Indian Society of Periodontology, Vol. 19, Issue 5, pp. 525-30, (2015) (PubMed).

Details zu MMP8

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Andere Bezeichnung: MMP8

Hintergrund:

Background: Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57 % identity with the deduced protein sequence for fibroblast collagenase with 72 % chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Gene Full Name: matrix metallopeptidase 8

Gen-ID: 4317

UniProt: P22894