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G-CSF ELISA Kit (Colony Stimulating Factor 3 (Granulocyte)) ELISA Kit

CSF3 Reaktivität: Human AA 31-204 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Produktnummer ABIN411275
$369.00
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 4 bis 6 Werktagen
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3)
    Bindungsspezifität
    AA 31-204
    Reaktivität
    • 8
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2-2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human G-CSF
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: T31-P204
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <4pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: T31-P204
    Featured
    Zu unserem meistverkauften CSF3 ELISA Kit
  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the IL-6 superfamily.

    Plattentyp
    Pre-coated
    Protokoll
    human G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for G-CSF has been precoated onto 96-well plates. Standards(E.coli, T31-P204) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human G-CSF amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human G-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human G-CSF standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 145, Standard deviation: 5.51, CV(%): 3.8
    • Sample 2: n=16, Mean(pg/ml): 727, Standard deviation: 30.53, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 1436, Standard deviation: 58.88, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 153, Standard deviation: 8.42, CV(%): 5.5
    • Sample 2: n=24, Mean(pg/ml): 718, Standard deviation: 46.67, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 1537, Standard deviation: 104.5, CV(%): 6.8
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3)
    Andere Bezeichnung
    CSF3 (CSF3 Produkte)
    Synonyme
    CSF3, G-CSF, gcsf, Csfg, MGI-IG, C17orf33, CSF3OS, GCSF, Gcsf, colony stimulating factor 3, colony stimulating factor 3 (granulocyte) a, colony stimulating factor 3 (granulocyte), CSF3, csf3a, Csf3
    Hintergrund

    Protein Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

    Background: Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

    Synonyms: Granulocyte colony-stimulating factor,G-CSF,Pluripoietin,Filgrastim,Lenograstim,CSF3,C17orf33, GCSF,

    Full Gene Name: Granulocyte colony-stimulating factor

    Cellular Localisation: Secreted.
    Gen-ID
    1440
    UniProt
    P09919
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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