IgG ELISA Kit

Produktnummer ABIN3199222

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human IgG.

Kurzübersicht für IgG ELISA Kit (ABIN3199222)

Target: IgG

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.13 ng/mL - 200 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails IgG ELISA Kit

Untere Nachweisgrenze: 3.13 ng/mL

Verwendungszweck: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IgG.

Analytische Methode: Quantitative

Spezifität: IgG ELISA Kit, human

Bestandteile:

• Plate
• Standard
• Diluent

Benötigtes Material: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Details zu IgG

Target: IgG

Substanzklasse: Antibody

Hintergrund: Immunoglobulin G (IgG) is a type of antibody. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. IgG is the main type of antibody found in blood and extracellular fluid allowing it to control infection of body tissues. IgG molecules are created and released by plasma B cells. IgG is the only isotype that has receptors to facilitate passage through the human placenta, thereby providing protection to the fetus in utero. IgG antibodies are extracted from donated blood plasma and used as a therapeutic known as intravenous immunoglobulin (IVIG). This is used to treat immune deficiencies, autoimmune disorders, and infections.

Gen-ID: 3500