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Asporin ELISA Kit

Human Asporin ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Asporin.
Produktnummer ABIN2945450

Kurzübersicht für Asporin ELISA Kit (ABIN2945450)

Target

Alle Asporin (ASPN) ELISA Kits anzeigen
Asporin (ASPN)

Reaktivität

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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

0.16 ng/mL - 10 ng/mL

Applikation

ELISA

Proben

Plasma, Serum
  • Untere Nachweisgrenze

    0.16 ng/mL

    Verwendungszweck

    Human Asporin (ASPN) ELISA Kit

    Analytische Methode

    Quantitative

    Sensitivität

    0.09 ng/mL

    Bestandteile

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Benötigtes Material

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Applikationshinweise

    Optimal dilutions/concentrations should be determined by the end user.

    Kommentare

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Plattentyp

    Pre-coated

    Testpräzision

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Asporin (ASPN) were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Asporin (ASPN) were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C

    Informationen zur Lagerung

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Haltbarkeit

    6 months
  • Target Alle Asporin (ASPN) ELISA Kits anzeigen

    Asporin (ASPN)

    Andere Bezeichnung

    Asporin

    Hintergrund

    Ensembl: ENSG00000106819

    UniProt Entry Name: ASPN_HUMAN

    Gen-ID

    54829

    HGNC

    14872

    OMIM

    603932

    UniProt

    Q9BXN1
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