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BNP-45 FLISA Kit

BNP-45 Reaktivität: Maus Fluorometric Competition ELISA
Produktnummer ABIN2871355
  • Target Alle BNP-45 Produkte
    BNP-45 (Brain Natriuretic Peptide 45 (BNP-45))
    Reaktivität
    • 2
    • 1
    • 1
    Maus
    Nachweismethode
    Fluorometric
    Methodentyp
    Competition ELISA
    Applikation
    ELISA
    Analytische Methode
    Quantitative
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
  • Target Alle BNP-45 Produkte
    BNP-45 (Brain Natriuretic Peptide 45 (BNP-45))
    Abstract
    BNP-45 Produkte
    Synonyme
    BNP FLISA Kit, Bnf FLISA Kit, natriuretic peptide B FLISA Kit, Nppb FLISA Kit
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