Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

BNP-45 FLISA Kit

Dieses Fluorometric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus BNP-45 in .
Produktnummer ABIN2871355

Kurzübersicht für BNP-45 FLISA Kit (ABIN2871355)

Target

Alle BNP-45 Produkte
BNP-45 (Brain Natriuretic Peptide 45 (BNP-45))

Reaktivität

  • 2
  • 1
  • 1
Maus

Nachweismethode

Fluorometric

Methodentyp

Competition ELISA

Applikation

ELISA
  • Analytische Methode

    Quantitative
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Plattentyp

    Pre-coated

    Protokoll

    The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C
  • Target Alle BNP-45 Produkte

    BNP-45 (Brain Natriuretic Peptide 45 (BNP-45))
Sie sind hier:
Chat with us!