RNA ChIP-IT Control Kit

Produktnummer ABIN2866108

Produktdetails

Reaktivität: Human

Applikation: Chromatin Immunoprecipitation (ChIP)

Marke: ChIP-IT®

Spezifität: The RNA ChIP-IT Control Kit - Human is quality-control tested to demonstrate that the positive and negative control antibodies are effective in performing RNA-chromatin immunoprecipitation.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

HeLa cells were grown, fixed and used to prepare chromatin as described in the RNA ChIP-IT Kit manual. RNA-ChIP reactions were then performed using 10 μl of Suz12 antibody and 10 μl of control rabbit IgG (0.2 μg/μl). The immunoprecipitated RNA and the control input RNA were then used in RT-PCR using lincSFPQ primer mix. Kit Components: 50 μl Suz12 rabbit polyclonal antibody 100 μl rabbit IgG (0.2 μg/μl) 400 μl lincSFPQ primer mix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The RNA-ChIP Control Kit - Human components are shipped on dry ice. The lincSFPQ primer mix, the Suz12 antibody and the control rabbit IgG should be stored at -20°C. This product is guaranteed for 6 months from date of receipt under the correct storage conditions. Aliquot the antibodies to avoid exposing to multiple freeze-thaw cycles.

Haltbarkeit: 6 months