Neuropilin 1 ELISA Kit

Produktnummer ABIN2859344

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus Neuropilin 1 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA).

Kurzübersicht für Neuropilin 1 ELISA Kit (ABIN2859344)

Target: Neuropilin 1 (NRP1)

Bindungsspezifität: AA 22-856

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 780-50.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Neuropilin 1 ELISA Kit

Untere Nachweisgrenze: 780 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Neuropilin-1

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: F22-P856

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <20pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: F22-P856

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the neuropilin family.

Tissue Specificity: Nervous system.

Plattentyp: Pre-coated

Protokoll: mouse Neuropilin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neuropilin-1 has been precoated onto 96-well plates. Standards(NSO, F22-P856) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Neuropilin-1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Neuropilin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Neuropilin-1 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 7.7, Standard deviation: 0.393, CV(%): 5.1
• Sample 2: n=16, Mean(ng/ml): 19, Standard deviation: 1.178, CV(%): 6.2
• Sample 3: n=16, Mean(ng/ml): 28, Standard deviation: 1.316, CV(%): 4.7,
• Sample 1: n=24, Mean(ng/ml): 8.3, Standard deviation: 0.465, CV(%): 5.6
• Sample 2: n=24, Mean(ng/ml): 25, Standard deviation: 1.85, CV(%): 7.4
• Sample 3: n=24, Mean(ng/ml): 34, Standard deviation: 1.802, CV(%): 5.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Neuropilin 1

Target: Neuropilin 1 (NRP1)

Andere Bezeichnung: NRP1

Hintergrund:

Protein Function: Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis (By similarity). .

Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

Synonyms: Neuropilin-1,A5 protein,CD304,Nrp1,Nrp,

Full Gene Name: Neuropilin-1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

Gen-ID: 18186

UniProt: P97333

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals