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Mesothelin ELISA Kit

MSLN Reaktivität: Human AA 296-580 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Produktnummer ABIN2859340
  • Target Alle Mesothelin (MSLN) ELISA Kits anzeigen
    Mesothelin (MSLN)
    Bindungsspezifität
    AA 296-580
    Reaktivität
    • 6
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156-10.000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Mesothelin
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: E296-G580
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E296-G580
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). .

    Plattentyp
    Pre-coated
    Protokoll
    human Mesothelin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Mesothelin has been precoated onto 96-well plates. Standards(NSO, E296-G580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Mesothelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Mesothelin amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Mesothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Mesothelin standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(ng/ml): 0.98, Standard deviation: 0.044, CV(%): 4.5
    • Sample 2: n=16, Mean(ng/ml): 2.73, Standard deviation: 0.098, CV(%): 3.6
    • Sample 3: n=16, Mean(ng/ml): 5.72, Standard deviation: 0.235, CV(%): 4.1,
    • Sample 1: n=24, Mean(ng/ml): 1.09, Standard deviation: 0.073, CV(%): 6.7
    • Sample 2: n=24, Mean(ng/ml): 3.16, Standard deviation: 0.167, CV(%): 5.3
    • Sample 3: n=24, Mean(ng/ml): 5.95, Standard deviation: 0.381, CV(%): 6.4
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle Mesothelin (MSLN) ELISA Kits anzeigen
    Mesothelin (MSLN)
    Andere Bezeichnung
    MSLN (MSLN Produkte)
    Synonyme
    MPF ELISA Kit, SMRP ELISA Kit, MSLN ELISA Kit, mesothelin ELISA Kit, MSLN ELISA Kit, Msln ELISA Kit, LOC611363 ELISA Kit, LOC100524016 ELISA Kit
    Hintergrund

    Protein Function: Membrane-anchored forms may play a role in cellular adhesion.

    Background: Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

    Synonyms: Mesothelin,CAK1 antigen,Pre-pro-megakaryocyte-potentiating factor,Megakaryocyte-potentiating factor,MPF,Mesothelin, cleaved form,MSLN,MPF,

    Full Gene Name: Mesothelin

    Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. Golgi apparatus.
    Gen-ID
    10232
    UniProt
    Q13421
    Pathways
    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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