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TIMP4 ELISA Kit

Dieses Maus TIMP4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus TIMP4 in Cell Culture Supernatant, Serum, Plasma (heparin) und Plasma (EDTA) zu quantifizieren.
Produktnummer ABIN2859339

Kurzübersicht für TIMP4 ELISA Kit (ABIN2859339)

Target

Alle TIMP4 ELISA Kits anzeigen
TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Bindungsspezifität

AA 30-224

Reaktivität

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Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

78-5.000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    78 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TIMP-4

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: C30-P224

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: C30-P224
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family.

    Tissue Specificity: Expressed in brain, heart, ovary and skeletal muscle.

    Plattentyp

    Pre-coated

    Protokoll

    mouse TIMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TIMP-4 has been precoated onto 96-well plates. Standards(NSO, C30-P224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TIMP-4 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 5,000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL mouse TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse TIMP-4 standard solution and each sample is measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 272, Standard deviation: 12.24, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 830, Standard deviation: 473.1, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 1934, Standard deviation: 98.6, CV(%): 5.1,
    • Sample 1: n=24, Mean(pg/ml): 296, Standard deviation: 22.5, CV(%): 7.6
    • Sample 2: n=24, Mean(pg/ml): 793, Standard deviation: 53.92, CV(%): 6.8
    • Sample 3: n=24, Mean(pg/ml): 1615, Standard deviation: 116.3, CV(%): 7.2

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle TIMP4 ELISA Kits anzeigen

    TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

    Andere Bezeichnung

    TIMP4

    Hintergrund

    Protein Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

    Background: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What’s more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

    Synonyms: Metalloproteinase inhibitor 4,Tissue inhibitor of metalloproteinases 4,TIMP-4,Timp4,

    Full Gene Name: Metalloproteinase inhibitor 4

    Cellular Localisation: Secreted.

    Gen-ID

    110595

    UniProt

    Q9JHB3
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