Osteoactivin ELISA Kit

Produktnummer ABIN2859337

Maus Osteoactivin ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus Osteoactivin.

Kurzübersicht für Osteoactivin ELISA Kit (ABIN2859337)

Target: Osteoactivin (GPNMB)

Bindungsspezifität: AA 23-502

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Osteoactivin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Osteoactivin/GPNMB

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: K23-N502

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: K23-N502

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the PMEL/NMB family.

Tissue Specificity: In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype. .

Plattentyp: Pre-coated

Protokoll: mouse Osteoactivin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Osteoactivin has been precoated onto 96-well plates. Standards(NSO, K23-N502) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Osteoactivin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Osteoactivin amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse Osteoactivin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Osteoactivin standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1174, Standard deviation: 64.57, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 3248, Standard deviation: 207.9, CV(%): 6.4
• Sample 3: n=16, Mean(pg/ml): 5532, Standard deviation: 271.1, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 1354, Standard deviation: 82.6, CV(%): 6.1
• Sample 2: n=24, Mean(pg/ml): 3426, Standard deviation: 246.7, CV(%): 7.2
• Sample 3: n=24, Mean(pg/ml): 5840, Standard deviation: 315.4, CV(%): 5.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Osteoactivin

Target: Osteoactivin (GPNMB)

Andere Bezeichnung: GPNMB

Hintergrund:

Protein Function: Could be a melanogenic enzyme. .

Background: Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis(soft callus) and osteogenesis(hard callus) compared to the intact femurs that is why Osteoactivin(OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Synonyms: Transmembrane glycoprotein NMB,DC-HIL,Dendritic cell-associated transmembrane protein,Osteoactivin,Gpnmb,Dchil, Hgfin, Nmb,

Full Gene Name: Transmembrane glycoprotein NMB

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Endomembrane system, Single-pass type I membrane protein . Cytoplasmic vesicle membrane, Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures.

Gen-ID: 93695

UniProt: Q99P91