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SHBG ELISA Kit

Dieses Human SHBG ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human SHBG zu quantifizieren.
Produktnummer ABIN2859324

Kurzübersicht für SHBG ELISA Kit (ABIN2859324)

Target

Alle SHBG ELISA Kits anzeigen
SHBG (Sex Hormone Binding Globulin (SHBG))

Bindungsspezifität

AA 30-402

Reaktivität

  • 6
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  • 3
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  • 1
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

0.031-2 nM/L

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    0.031 nM/L

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human SHBG

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: NSO
    Immunogen sequence: L30-H402

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <0.001nmol/L

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: L30-H402
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Contains 2 laminin G-like domains.

    Tissue Specificity: Isoform 1 and isoform 2 are present in liver and testis.

    Plattentyp

    Pre-coated

    Protokoll

    human SHBG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SHBG has been precoated onto 96-well plates. Standards(NSO, L30-H402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SHBG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SHBG amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 2nmol/L, 1nmol/L, 0.5nmol/L, 0.25nmol/L, 0.125nmol/L, 0.062nmol/L, 0.031nmol/L human SHBG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human SHBG standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(nmol/L): 0.19, Standard deviation: 0.009, CV(%): 4.5
    • Sample 2: n=16, Mean(nmol/L): 0.76, Standard deviation: 0.043, CV(%): 5.6
    • Sample 3: n=16, Mean(nmol/L): 1.4, Standard deviation: 0.095, CV(%): 6.8,
    • Sample 1: n=24, Mean(nmol/L): 0.24, Standard deviation: 0.014, CV(%): 5.7
    • Sample 2: n=24, Mean(nmol/L): 0.85, Standard deviation: 0.051, CV(%): 6
    • Sample 3: n=24, Mean(nmol/L): 1.9, Standard deviation: 0.137, CV(%):7.2

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle SHBG ELISA Kits anzeigen

    SHBG (Sex Hormone Binding Globulin (SHBG))

    Andere Bezeichnung

    SHBG

    Hintergrund

    Protein Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

    Background: Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12-p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

    Synonyms: Sex hormone-binding globulin,SHBG,Sex steroid-binding protein,SBP,Testis-specific androgen-binding protein,ABP,Testosterone-estradiol-binding globulin,TeBG,Testosterone-estrogen-binding globulin,SHBG,

    Full Gene Name: Sex hormone-binding globulin

    Cellular Localisation: Secreted . In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis..

    Gen-ID

    6462

    UniProt

    P04278

    Pathways

    Hormone Transport
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