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HGF ELISA Kit

HGF Reaktivität: Maus AA 33-495 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Serum
Produktnummer ABIN2859322
  • Target Alle HGF ELISA Kits anzeigen
    HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
    Bindungsspezifität
    AA 33-495, AA 496-728
    Reaktivität
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse HGF
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: Q33-R495 (i±)&V496-L728 (i²)
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q33-R495 (alpha)&V496-L728 (beta)
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

    Plattentyp
    Pre-coated
    Protokoll
    mouse HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for HGF has been precoated onto 96-well plates. Standards(NSO,Q33-R495 (α) & V496 - L728 (β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse HGF amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse HGF standard solution and each sample is measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 427, Standard deviation: 17.93, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 1523, Standard deviation: 76.15, CV(%): 5.0
    • Sample 3: n=16, Mean(pg/ml): 3342, Standard deviation: 187.15, CV(%): 5.6,
    • Sample 1: n=24, Mean(pg/ml): 501, Standard deviation: 37.58, CV(%): 7.5
    • Sample 2: n=24, Mean(pg/ml): 1756, Standard deviation: 136.97, CV(%): 7.8
    • Sample 3: n=24, Mean(pg/ml): 3471, Standard deviation: 284.62, CV(%): 8.2
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Secchiero, Corallini, Zavan, Tripodo, Vindigni, Zauli: "Mesenchymal stem cells display hepato-protective activity in lymphoma bearing xenografts." in: Investigational new drugs, Vol. 30, Issue 2, pp. 803-7, (2012) (PubMed).

  • Target Alle HGF ELISA Kits anzeigen
    HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
    Andere Bezeichnung
    HGF (HGF Produkte)
    Synonyme
    DFNB39 ELISA Kit, F-TCF ELISA Kit, HGFB ELISA Kit, HPTA ELISA Kit, SF ELISA Kit, HGF ELISA Kit, C230052L06Rik ELISA Kit, HGF/SF ELISA Kit, NK1 ELISA Kit, NK2 ELISA Kit, SF/HGF ELISA Kit, hepatocyte growth factor ELISA Kit, HGF ELISA Kit, Hgf ELISA Kit
    Hintergrund

    Protein Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. .

    Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

    Synonyms: Hepatocyte growth factor,Hepatopoietin-A,Scatter factor,SF,Hepatocyte growth factor alpha chain,Hepatocyte growth factor beta chain,Hgf,

    Full Gene Name: Hepatocyte growth factor

    Gen-ID
    15234
    UniProt
    Q08048
    Pathways
    RTK Signalweg, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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