TGFBI ELISA Kit

Produktnummer ABIN2859321

Produktdetails TGFBI ELISA Kit

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Bindungsspezifität: AA 24-683

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse beta IG-H3/TGFBI

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: NSO, G24-H683

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <5pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: G24-H683

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Expressed in heart, kidney, liver, skeletal muscle, testis, thyroid and uterus (PubMed:8024701). .

Plattentyp: Pre-coated

Protokoll: mouse beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for beta IG-H3 has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse beta IG-H3 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse βIG-H3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse βIG-H3 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 447, Standard deviation: 28.61, CV(%): 6.4
• Sample 2: n=16, Mean(pg/ml): 1253, Standard deviation: 53.88, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 2346, Standard deviation: 115, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 450, Standard deviation: 39.15, CV(%): 8.7
• Sample 2: n=24, Mean(pg/ml): 1238, Standard deviation: 64.38, CV(%): 5.2
• Sample 3: n=24, Mean(pg/ml): 2625, Standard deviation: 144.4, CV(%): 5.5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für TGFBI ELISA Kit (ABIN2859321)

Ge, Yu, Liu, Cong, Liu, Wang, Zhou, Lin: "Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3498-506, (2016) (PubMed).

Details zu TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Andere Bezeichnung: TGFBI (TGFBI Produkte)

Hintergrund:

Protein Function: Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity). .

Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Synonyms: Transforming growth factor-beta-induced protein ig-h3,Beta ig-h3,Tgfbi,

Full Gene Name: Transforming growth factor-beta-induced protein ig-h3

Cellular Localisation: Secreted . Secreted, extracellular space, extracellular matrix . May be associated both with microfibrils and with the cell surface..

Gen-ID: 21810

UniProt: P82198