IL-25 ELISA Kit

Produktnummer ABIN2859300

Produktdetails IL-25 ELISA Kit

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Bindungsspezifität: AA 33-177

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17E/IL-25

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: Y33-G177

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: Y33-G177

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the IL-17 family.

Tissue Specificity: Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

Plattentyp: Pre-coated

Protokoll: human IL-17E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been precoated onto 96-well plates. Standards(E.coli, Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17E amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-17E standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human IL-17E standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 621, Standard deviation: 24.2, CV(%): 3.9
• Sample 2: n=16, Mean(pg/ml): 1969, Standard deviation: 90.6, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 2795, Standard deviation: 150.9, CV(%): 5.4,
• Sample 1: n=24, Mean(pg/ml): 730, Standard deviation: 30.7, CV(%): 4.2
• Sample 2: n=24, Mean(pg/ml): 2103, Standard deviation: 119.9, CV(%): 5.7
• Sample 3: n=24, Mean(pg/ml): 3065, Standard deviation: 208.4, CV(%): 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für IL-25 ELISA Kit (ABIN2859300)

Meng, Shen, Shen, Zhou, Shen, Das: "Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro." in: Lipids in health and disease, Vol. 12, pp. 160, (2014) (PubMed).

Details zu IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Andere Bezeichnung: IL25 (IL25 Produkte)

Synonyme: IL17E ELISA Kit, IL-17e ELISA Kit, Il17e ELISA Kit, IL25 ELISA Kit, RGD1561632 ELISA Kit, interleukin 25 ELISA Kit, IL25 ELISA Kit, Il25 ELISA Kit

Hintergrund:

Protein Function: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Background: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-i°B activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

Synonyms: Interleukin-25,IL-25,Interleukin-17E,IL-17E,IL25,IL17E,UNQ3120/PRO10272,

Full Gene Name: Interleukin-25

Cellular Localisation: Secreted.

Gen-ID: 64806

UniProt: Q9H293