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IL17C ELISA Kit

Maus IL17C ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus IL17C.
Produktnummer ABIN2859298

Kurzübersicht für IL17C ELISA Kit (ABIN2859298)

Target

Alle IL17C ELISA Kits anzeigen
IL17C (Interleukin 17C (IL17C))

Bindungsspezifität

AA 17-194

Reaktivität

  • 3
  • 3
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

125-8000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
  • Untere Nachweisgrenze

    125 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17C

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    Expression system for standard: E.coli
    Immunogen sequence: D17-Q194

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: D17-Q194
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Sequence similarities: Belongs to the IL-17 family.

    Tissue Specificity: Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes. .

    Plattentyp

    Pre-coated

    Protokoll

    mouse IL-17C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17C has been precoated onto 96-well plates. Standards(E.coli, D17-Q194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17C amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IL-17C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17C standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(ng/ml): 1.3, Standard deviation: 0.053, CV(%): 4.1
    • Sample 2: n=16, Mean(ng/ml): 3.8, Standard deviation: 0.186, CV(%): 4.9
    • Sample 3: n=16, Mean(ng/ml): 5.7, Standard deviation: 0.296, CV(%): 5.2,
    • Sample 1: n=24, Mean(ng/ml): 1.6, Standard deviation: 0.086, CV(%): 5.4
    • Sample 2: n=24, Mean(ng/ml): 4.5, Standard deviation: 0.279, CV(%): 6.2
    • Sample 3: n=24, Mean(ng/ml): 6.3, Standard deviation: 0.422, CV(%): 6.7

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle IL17C ELISA Kits anzeigen

    IL17C (Interleukin 17C (IL17C))

    Andere Bezeichnung

    IL17C

    Hintergrund

    Protein Function: Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NFKB and MAPK pathways. Acts synergically with IL22, TNF and IL1B in inducing antibacterial peptides. May have protective function by maintaining epithelial homeostasis after an inflammatory challenge, such as that caused in the intestine by dextran sulfate sodium in a colitis model. May also promote an inflammatory phenotype, such as skin in a psoriasis model. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases, such as autoimmune encephalitis. .

    Background: IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

    Synonyms: Interleukin-17C,Il-17c,Cytokine CX2,Il17c,

    Full Gene Name: Interleukin-17C

    Cellular Localisation: Secreted.

    Gen-ID

    234836

    UniProt

    Q8K4C5

    Pathways

    Cellular Response to Molecule of Bacterial Origin
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