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SDC4 ELISA Kit

SDC4 Reaktivität: Human AA 19-145 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Produktnummer ABIN2859227
  • Target Alle SDC4 ELISA Kits anzeigen
    SDC4 (Syndecan 4 (SDC4))
    Bindungsspezifität
    AA 19-145
    Reaktivität
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    62.5-4000 pg/mL
    Untere Nachweisgrenze
    62.5 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Syndecan-4
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: NSO
    Immunogen sequence: E19-E145
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E19-E145
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Kommentare

    Tissue Specificity: Expressed in epithelial and fibroblastic cells. .

    Plattentyp
    Pre-coated
    Protokoll
    human Syndecan-4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Syndecan-4 has been precoated onto 96-well plates. Standards(NSO, E19-E145) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-4 amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Syndecan-4 standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 630, Standard deviation: 26.5, CV(%): 4.2
    • Sample 2: n=16, Mean(pg/ml): 1447, Standard deviation: 83.9, CV(%): 5.8
    • Sample 3: n=16, Mean(pg/ml): 2135, Standard deviation: 100.3, CV(%): 4.7,
    • Sample 1: n=24, Mean(pg/ml): 718, Standard deviation: 35.2, CV(%): 4.9
    • Sample 2: n=24, Mean(pg/ml): 1604, Standard deviation: 104.3, CV(%): 6.5
    • Sample 3: n=24, Mean(pg/ml): 2369, Standard deviation: 120.8, CV(%): 5.1
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Target Alle SDC4 ELISA Kits anzeigen
    SDC4 (Syndecan 4 (SDC4))
    Andere Bezeichnung
    SDC4 (SDC4 Produkte)
    Synonyme
    SYND4 ELISA Kit, AA959608 ELISA Kit, AW108331 ELISA Kit, Synd4 ELISA Kit, ryudocan ELISA Kit, syndecan-4 ELISA Kit, RATRYUDOCA ELISA Kit, RYUDOCA ELISA Kit, SDC4 ELISA Kit, sdc4l ELISA Kit, wu:fc47g09 ELISA Kit, Syndecan-4 ELISA Kit, amphiglycan ELISA Kit, syn-4 ELISA Kit, syn4 ELISA Kit, syndecan 4 ELISA Kit, syndecan 4 S homeolog ELISA Kit, syndecan 4 L homeolog ELISA Kit, SDC4 ELISA Kit, Sdc4 ELISA Kit, sdc4 ELISA Kit, sdc4.S ELISA Kit, sdc4.L ELISA Kit
    Hintergrund

    Protein Function: Cell surface proteoglycan that bears heparan sulfate.

    Background: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.

    Synonyms: Syndecan-4,SYND4,Amphiglycan,Ryudocan core protein,SDC4,

    Full Gene Name: Syndecan-4

    Cellular Localisation: Isoform 1: Membrane, Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form..
    Gen-ID
    6385
    UniProt
    P31431
    Pathways
    Glycosaminoglycan Metabolic Process
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