Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CXCL5 ELISA Kit

Dieses Ratte CXCL5 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte CXCL5 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) und Tissue Homogenate zu quantifizieren.
Produktnummer ABIN2859210

Kurzübersicht für CXCL5 ELISA Kit (ABIN2859210)

Target

Alle CXCL5 ELISA Kits anzeigen
CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

Bindungsspezifität

AA 38-130

Reaktivität

  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Ratte

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6-1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Tissue Homogenate
  • Untere Nachweisgrenze

    15.6 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL5/LIX

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    E.coli, A38-Q130

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <1pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: E.coli
    Immunogen sequence: A38-Q130
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Plattentyp

    Pre-coated

    Protokoll

    rat CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, A38-Q130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL5 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin, EDTA, citrate) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CXCL5 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(pg/ml): 75.4, Standard deviation: 4.1, CV(%): 5.4
    • Sample 2: n=16, Mean(pg/ml): 221, Standard deviation: 13.7, CV(%): 6.2
    • Sample 3: n=16, Mean(pg/ml): 546, Standard deviation: 28.4, CV(%): 5.2,
    • Sample 1: n=24, Mean(pg/ml): 98.4, Standard deviation: 6, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 285, Standard deviation: 20.52, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 534, Standard deviation: 31.5, CV(%): 5.9

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle CXCL5 ELISA Kits anzeigen

    CXCL5 (Chemokine (C-X-C Motif) Ligand 5 (CXCL5))

    Andere Bezeichnung

    CXCL5

    Hintergrund

    Protein Function: May participate in the recruitment of inflammatory cells by injured or infected tissue. .

    Background: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

    Synonyms: C-X-C motif chemokine 5,Cytokine LIX,Small-inducible cytokine B5,Cxcl5,Scyb5,

    Full Gene Name: C-X-C motif chemokine 5

    Cellular Localisation: Secreted.

    Gen-ID

    105988538

    UniProt

    P97885

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity
Sie sind hier:
Chat with us!