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RPS6 ELISA Kit

Dieses Human, Maus und Ratte RPS6 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human, Maus und Ratte RPS6 in Cell Lysate und Tissue Lysate zu quantifizieren.
Produktnummer ABIN2748616

Kurzübersicht für RPS6 ELISA Kit (ABIN2748616)

Target

Alle RPS6 ELISA Kits anzeigen
RPS6 (Ribosomal Protein S6 (RPS6))

Bindungsspezifität

pSer235, pSer236, total

Reaktivität

  • 7
  • 6
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Lysate, Tissue Lysate
  • Verwendungszweck

    Human/Mouse/Rat Phospho-RPS6 (S235/S236) and Total RPS6 ELISA Kit. This assay semi-quantitatively measures phophorylated RPS6 (Ser235/S236) and Total RPS6 in lysate samples.

    Analytische Methode

    Semi-Quantitative

    Spezifität

    The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-RPS6 (pSer235/Ser236) and total RPS6.

    Produktmerkmale

    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample

    Benötigtes Material

    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Testdurchführung

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    Haltbarkeit

    6 months
  • Target Alle RPS6 ELISA Kits anzeigen

    RPS6 (Ribosomal Protein S6 (RPS6))

    Andere Bezeichnung

    RPS6

    Hintergrund

    Ribosomal protein S6 phosphorylated at S235/S236 and total RPS6

    Gen-ID

    6194

    UniProt

    P62753

    Pathways

    Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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