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P-Cadherin ELISA Kit

CDH3 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.041-10 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2748467
  • Target Alle P-Cadherin (CDH3) ELISA Kits anzeigen
    P-Cadherin (CDH3)
    Reaktivität
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.041-10 ng/mL
    Untere Nachweisgrenze
    0.041 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Rat P-Cadherin ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB (TNFRSF9/CD137), Activin A, Adiponectin (ACRP30), CD80 (B7-1), CD48 (SLAMF2), CTACK, Decorin, Eotaxin-1, EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1 (GDNFR), gp130, HGF, IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, Prolactin, RANTES, SCF, TIM-1 (KIM-1), TIMP-2, TREM-1, TWEAK R.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples4 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle P-Cadherin (CDH3) ELISA Kits anzeigen
    P-Cadherin (CDH3)
    Andere Bezeichnung
    P-Cadherin (CDH3 Produkte)
    Synonyme
    CDH3 ELISA Kit, Cadherin-3 ELISA Kit, AI385538 ELISA Kit, Cadp ELISA Kit, Cdhp ELISA Kit, P-cadherin ELISA Kit, Pcad ELISA Kit, CDHP ELISA Kit, HJMD ELISA Kit, PCAD ELISA Kit, E-Cad ELISA Kit, E-cadherin ELISA Kit, EP-cadherin ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, cdh1 ELISA Kit, cdh1-b ELISA Kit, cdh3 ELISA Kit, cdhc-A ELISA Kit, cdhp ELISA Kit, hjmd ELISA Kit, l-cam ELISA Kit, lcam ELISA Kit, pcad ELISA Kit, uvo ELISA Kit, uvomorulin ELISA Kit, xb-cad ELISA Kit, xcdh1 ELISA Kit, CDH15 ELISA Kit, K-CAM ELISA Kit, cadherin 3 ELISA Kit, cadherin 3 L homeolog ELISA Kit, Cadherin-3 ELISA Kit, CDH3 ELISA Kit, Cdh3 ELISA Kit, cdh3.L ELISA Kit, cdh-3 ELISA Kit
    Hintergrund
    P-Cadherin (CDH3 / Cadherin-3)
    UniProt
    Q9JIV6
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