Neuropilin 1 ELISA Kit

Produktnummer ABIN2748424

Das Ratte Neuropilin 1 ELISA Kit (ABIN2748424) ist ein Colorimetric ELISA Kit zur Detektion von Ratte Neuropilin 1.

Kurzübersicht für Neuropilin 1 ELISA Kit (ABIN2748424)

Target: Neuropilin 1 (NRP1)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.03 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails Neuropilin 1 ELISA Kit

Untere Nachweisgrenze: 0.03 ng/mL

Verwendungszweck: The Rat Neuropilin-1 ELISA Kit is a pre-coated sandwich-based assay kit designed to quantitatively measure Neuropilin-1 (NRP1) levels in rat cell culture media, plasma, and serum.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects rat NRP1. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution
• Assay Diluent(s)
• Adhesive Sealing Film

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare standard or sample dilutions
• Plastic wrap

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Neuropilin 1

Target: Neuropilin 1 (NRP1)

Andere Bezeichnung: Neuropilin-1

Hintergrund:

Gene Symbols: NRP1

Protein Name (Synonyms): Neuropilin-1 (Vascular endothelial cell growth factor 165 receptor) (CD antigen CD304)

Gen-ID: 246331

UniProt: Q9QWJ9

Pathways: Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals