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BCAM ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Rhesus Monkey BCAM in Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate.
Produktnummer ABIN2747976

Kurzübersicht für BCAM ELISA Kit (ABIN2747976)

Target

Alle BCAM ELISA Kits anzeigen
BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

Reaktivität

  • 4
  • 2
  • 1
  • 1
Rhesusaffen

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • Verwendungszweck

    Custom Rhesus Macaque Basal Cell Adhesion Molecule (BCAM) ELISA Kit.

    Analytische Methode

    Quantitative

    Spezifität

    The antibody pair provided in this kit recognizes Rhesus Macaque Basal Cell Adhesion Molecule (BCAM)

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target Alle BCAM ELISA Kits anzeigen

    BCAM (Basal Cell Adhesion Molecule (Lutheran Blood Group) (BCAM))

    Andere Bezeichnung

    BCAM

    Hintergrund

    Basal Cell Adhesion Molecule (BCAM)

    UniProt

    H9Z2N4
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