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WNK1 ELISA Kit

WNK1 Reaktivität: Human pThr60 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN2747914
  • Target Alle WNK1 ELISA Kits anzeigen
    WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))
    Bindungsspezifität
    pThr60, total
    Reaktivität
    • 4
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-WNK1 (T60) & Total WNK1 ELISA Kit. This assay semi-quantitatively measures phophorylated WNK1 (Thr60) & Total WNK1 in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human WNK1 phosphorylated at site Threonine-60 and total WNK1.
    Produktmerkmale
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product WNK1 ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle WNK1 ELISA Kits anzeigen
    WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))
    Andere Bezeichnung
    WNK1 (WNK1 Produkte)
    Synonyme
    HSAN2 ELISA Kit, HSN2 ELISA Kit, KDP ELISA Kit, PRKWNK1 ELISA Kit, PSK ELISA Kit, p65 ELISA Kit, WNK1 ELISA Kit, kdp ELISA Kit, psk ELISA Kit, hsn2 ELISA Kit, hsan2 ELISA Kit, pha2c ELISA Kit, prkwnk1 ELISA Kit, 6430573H23Rik ELISA Kit, EG406236 ELISA Kit, Hsn2 ELISA Kit, Prkwnk1 ELISA Kit, mKIAA0344 ELISA Kit, ATWNK1 ELISA Kit, T9J14.14 ELISA Kit, T9J14_14 ELISA Kit, ZIK4 ELISA Kit, with no lysine (K) kinase 1 ELISA Kit, WNK lysine deficient protein kinase 1 ELISA Kit, WNK lysine deficient protein kinase 1 L homeolog ELISA Kit, serine/threonine-protein kinase WNK1 ELISA Kit, with no lysine (K) kinase 1 ELISA Kit, WNK1 ELISA Kit, wnk1.L ELISA Kit, wnk1 ELISA Kit, LOC100056745 ELISA Kit, Wnk1 ELISA Kit
    Hintergrund
    WNK Lysine-deficient Protein Kinase 1 (WNK1, KDP, PHA2C, PRKWNK1) phosphorylated at Threonine-60 & total WNK1
    Gen-ID
    65125
    UniProt
    Q9H4A3
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