MFGE8 ELISA Kit
Kurzübersicht für MFGE8 ELISA Kit (ABIN2708573)
Target
Alle MFGE8 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 15.6 pg/mL
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Verwendungszweck
- Sandwich ELISA for quantitative detection of Mouse MFG-E8 concentrations.
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Analytische Methode
- Quantitative
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Sensitivität
- 6 pg/mL
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Bestandteile
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Benötigtes Material
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA Kit
MFGE8 Reaktivität: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Ratte Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Human Colorimetric Sandwich ELISA 781.25 pg/mL - 50000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Human Colorimetric Sandwich ELISA 0.1-25 ng/mL Cell Culture Supernatant, Plasma, Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Human AA 24-387 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Maus AA 23-463 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA KitMFGE8 Reaktivität: Maus Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Probenmenge
- 100 μL
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Plattentyp
- Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid freeze/thaw cycles
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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Haltbarkeit
- 6-12 months
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- MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))
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Andere Bezeichnung
- MFG-E8
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Hintergrund
- Alternative Names: Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, MFG-E8, SED1
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Gen-ID
- 17304
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UniProt
- P21956
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Pathways
- SARS-CoV-2 Protein Interaktom
Target Alle MFGE8 ELISA Kits anzeigen
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