SFTPD ELISA Kit
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- Target Alle SFTPD ELISA Kits anzeigen
- SFTPD (Surfactant Protein D (SFTPD))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.12-30 ng/mL
- Untere Nachweisgrenze
- 0.12 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Human SP-D ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Proben
- Cell Culture Supernatant, Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- The antibody pair provided in this kit recognizes Human Surfactant Pulmonary Associated Protein D (SFTPD)
- Sensitivität
- 0.12 ng/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples2 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle SFTPD ELISA Kits anzeigen
- SFTPD (Surfactant Protein D (SFTPD))
- Andere Bezeichnung
- SP-D (SFTPD Produkte)
- Synonyme
- COLEC7 ELISA Kit, PSP-D ELISA Kit, SFTP4 ELISA Kit, SP-D ELISA Kit, BDE ELISA Kit, BDSD ELISA Kit, HOX4I ELISA Kit, SPD ELISA Kit, AI573415 ELISA Kit, Sftp4 ELISA Kit, surfactant protein D ELISA Kit, homeobox D13 ELISA Kit, surfactant associated protein D ELISA Kit, SFTPD ELISA Kit, HOXD13 ELISA Kit, Sftpd ELISA Kit, SP-D ELISA Kit
- Hintergrund
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Gene Names: SFTPD COLEC7 PSPD SFTP4
Protein names: Pulmonary surfactant-associated protein D (PSP-D) (SP-D) (Collectin-7) (Lung surfactant protein D)
- Gen-ID
- 6441
- UniProt
- P35247
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