Resistin ELISA Kit
Kurzübersicht für Resistin ELISA Kit (ABIN2703440)
Target
Alle Resistin (RETN) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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    - 
                                            Untere Nachweisgrenze
- 16 pg/mL
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                                            Verwendungszweck
- Canine Resistin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
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                                            Analytische Methode
- Quantitative
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                                            Spezifität
- The antibody pair provided in this kit recognizes Canine Resistin (RETN)
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                                            Sensitivität
- 16 pg/mL
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                                            Produktmerkmale
- 
                        - Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
 
- 
                                            Bestandteile
- 
                        - Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
 
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                                            Benötigtes Material
- 
                        - Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
 
 
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- 
    
- 
    - 
                                            Applikationshinweise
- Recommended Dilution for serum and plasma samples10 fold
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                                            Probenmenge
- 100 μL
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                                            Plattentyp
- Pre-coated
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                                            Protokoll
- 
                        - Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
 
- 
                                            Beschränkungen
- Nur für Forschungszwecke einsetzbar
 
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- 
    - 
                                            Lagerung
- -20 °C
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                                            Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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                                            Haltbarkeit
- 6 months
 
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- 
    - Resistin (RETN)
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                                            Andere Bezeichnung
- Resistin
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                                            Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
 Target Alle Resistin (RETN) ELISA Kits anzeigen
- 
                    
 
                                     
                                     
                                    