DLK1 ELISA Kit

Produktnummer ABIN2703419

Human DLK1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human DLK1 und es wurde in 1 Publikation zitiert.

Kurzübersicht für DLK1 ELISA Kit (ABIN2703419)

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.4-100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails DLK1 ELISA Kit

Untere Nachweisgrenze: 0.4 ng/mL

Verwendungszweck: Human Pref-1 (DLK-1/FA1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM-12, ADAM-8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58, CD84, CD99, CEACAM-5, Coagulation Factor XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein-2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, I-COS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin-3, Siglec-10, SLAM, SP-D, Syndecan-4,Testican 2, TIM-3, TLR4, TRAIL, ULBP-1.

Sensitivität: 0.4 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Referenzen für DLK1 ELISA Kit (ABIN2703419)

Schrey, Wurst, Ebert, Kralisch, Drewlo, Stepan, Lössner, Platz, Kratzsch, Stumvoll, Fasshauer: "The adipokine preadipocyte factor-1 is downregulated in preeclampsia and expressed in placenta." in: Cytokine, Vol. 75, Issue 2, pp. 338-43, (2015) (PubMed).

Details zu DLK1

Target: DLK1 (delta-Like 1 Homolog (Drosophila) (DLK1))

Andere Bezeichnung: Pref-1

Hintergrund: Gene Names: DLK1 DLK
Protein names: Protein delta homolog 1 (DLK-1) (pG2) [Cleaved into: Fetal antigen 1 (FA1)]

Gen-ID: 8788

UniProt: P80370