Osteocalcin ELISA Kit

Produktnummer ABIN2703384

Produktdetails Osteocalcin ELISA Kit

Target: Osteocalcin (BGLAP)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.4-100 ng/mL

Untere Nachweisgrenze: 0.4 ng/mL

Applikation: ELISA

Verwendungszweck: Human Osteocalcin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human Osteocalcin. Other species not determined.

Sensitivität: 0.4 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples5 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Osteocalcin

Target: Osteocalcin (BGLAP)

Andere Bezeichnung: Osteocalcin (BGLAP Produkte)

Synonyme: BGP ELISA Kit, OC ELISA Kit, OCN ELISA Kit, Bglap1 ELISA Kit, Bglap2 ELISA Kit, OG1 ELISA Kit, mOC-A ELISA Kit, Bgp ELISA Kit, Bgpr ELISA Kit, Bgpra ELISA Kit, bgp ELISA Kit, LOC792433 ELISA Kit, ocn ELISA Kit, GLA ELISA Kit, BGLAP ELISA Kit, PMF1 ELISA Kit, bone gamma-carboxyglutamate protein ELISA Kit, bone gamma carboxyglutamate protein ELISA Kit, bone gamma-carboxyglutamate protein S homeolog ELISA Kit, bone gamma-carboxyglutamate (gla) protein ELISA Kit, osteocalcin ELISA Kit, polyamine-modulated factor 1 ELISA Kit, BGLAP ELISA Kit, Bglap ELISA Kit, bglap.S ELISA Kit, bglap ELISA Kit, ocn ELISA Kit, LOC100529177 ELISA Kit, LOC100146589 ELISA Kit

Hintergrund: Gene Names: BGLAP
Protein names: Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)

Gen-ID: 632

UniProt: P02818