L1CAM ELISA Kit

Produktnummer ABIN2703287

Human L1CAM ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human L1CAM.

Kurzübersicht für L1CAM ELISA Kit (ABIN2703287)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.8-200 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails L1CAM ELISA Kit

Untere Nachweisgrenze: 0.8 pg/mL

Verwendungszweck: Human L1CAM (NCAM-L1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes human L1CAM. Other species not yet determined.

Sensitivität: 0.8 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples5,000,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

For example, add 2 μL of serum or plasma into a tube with 198 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 198 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample. Mix through and then pipette 2 μL of prepared 10,000-fold diluted sample into a tube with 998 μL 1x Assay Diluent to prepare a final 5,000,000 fold diluted sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Andere Bezeichnung: L1CAM

Hintergrund: Gene Names: L1CAM CAML1 MIC5
Protein names: Neural cell adhesion molecule L1 (N-CAM-L1) (NCAM-L1) (CD antigen CD171)

Gen-ID: 3897

UniProt: P32004

Pathways: Synaptic Membrane