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IL-37 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-37.
Produktnummer ABIN2703198

Kurzübersicht für IL-37 ELISA Kit (ABIN2703198)

Target

Alle IL-37 (IL37) ELISA Kits anzeigen
IL-37 (IL37) (Interleukin 37 (IL37))

Reaktivität

  • 4
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

80-20000 pg/mL

Applikation

ELISA

Proben

Plasma, Cell Culture Supernatant, Serum
  • Untere Nachweisgrenze

    80 pg/mL

    Verwendungszweck

    Human IL-37 (IL-1 F7) ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Analytische Methode

    Quantitative

    Spezifität

    This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F8 (IL-36 beta), IL-1F9 (IL-36 gamma), IL-1F10 (IL-38), IL-1F11 (IL-33)

    Sensitivität

    80 pg/mL

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise

    Recommended Dilution for serum and plasma samples2 fold

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target Alle IL-37 (IL37) ELISA Kits anzeigen

    IL-37 (IL37) (Interleukin 37 (IL37))

    Andere Bezeichnung

    IL-1F7

    Hintergrund

    Gene Names: IL37 FIL1Z IL1F7 IL1H4 IL1RP1
    Protein names: Interleukin-37 (FIL1 zeta) (IL-1X) (Interleukin-1 family member 7) (IL-1F7) (Interleukin-1 homolog 4) (IL-1H) (IL-1H4) (Interleukin-1 zeta) (IL-1 zeta) (Interleukin-1-related protein) (IL-1RP1) (Interleukin-23) (IL-37)

    Gen-ID

    27178

    UniProt

    Q9NZH6
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