IL-37 ELISA Kit

Produktnummer ABIN2703198

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-37.

Kurzübersicht für IL-37 ELISA Kit (ABIN2703198)

Target: IL-37 (IL37) (Interleukin 37 (IL37))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 80-20000 pg/mL

Applikation: ELISA

Proben: Plasma, Cell Culture Supernatant, Serum

Produktdetails IL-37 ELISA Kit

Untere Nachweisgrenze: 80 pg/mL

Verwendungszweck: Human IL-37 (IL-1 F7) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F8 (IL-36 beta), IL-1F9 (IL-36 gamma), IL-1F10 (IL-38), IL-1F11 (IL-33)

Sensitivität: 80 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu IL-37

Target: IL-37 (IL37) (Interleukin 37 (IL37))

Andere Bezeichnung: IL-1F7

Hintergrund: Gene Names: IL37 FIL1Z IL1F7 IL1H4 IL1RP1
Protein names: Interleukin-37 (FIL1 zeta) (IL-1X) (Interleukin-1 family member 7) (IL-1F7) (Interleukin-1 homolog 4) (IL-1H) (IL-1H4) (Interleukin-1 zeta) (IL-1 zeta) (Interleukin-1-related protein) (IL-1RP1) (Interleukin-23) (IL-37)

Gen-ID: 27178

UniProt: Q9NZH6