GREM1 ELISA Kit

Produktnummer ABIN2703067

Dieses Maus GREM1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus GREM1 zu quantifizieren.

Kurzübersicht für GREM1 ELISA Kit (ABIN2703067)

Target: GREM1 (Gremlin 1 (GREM1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.8-200 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails GREM1 ELISA Kit

Untere Nachweisgrenze: 0.8 ng/mL

Verwendungszweck: Mouse Gremlin-1 ELISA Kit for cell culture supernatants, EDTA treated plasma, and serum samples. Heparin and citrate are not recommended.

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS1, Adiponectin, ANG-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, IFN-gamma R1, IL-17B, IL-17B R, IL-22, MIP-1 beta, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGF beta-1, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2.

Sensitivität: 0.8 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu GREM1

Target: GREM1 (Gremlin 1 (GREM1))

Andere Bezeichnung: Gremlin-1

Hintergrund: Gene Names: Grem1 Cktsf1b1 Drm
Protein names: Gremlin-1 (Cysteine knot superfamily 1, BMP antagonist 1) (Down-regulated in Mos-transformed cells protein)

Gen-ID: 23892

UniProt: O70326

Pathways: Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location