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Tissue factor ELISA Kit
F3
Reaktivität: Maus
Colorimetric
Sandwich ELISA
1.6-400 ng/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702922
Produktdetails Tissue factor ELISA Kit
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Target
Alle Tissue factor (F3) ELISA Kits anzeigen
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Reaktivität
Alle Reaktivitäten für Tissue factor ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
1.6-400 ng/mL
Untere Nachweisgrenze
1.6 ng/mL
Applikation
ELISA
Verwendungszweck
Mouse Coagulation Factor III (Tissue Factor/Thromboplastin) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Mouse Coagulation Factor III (F3 / Tissue Factor / Thromboplastin)
Sensitivität
1.6 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu Tissue factor
(ausblenden)
Target
Alle Tissue factor (F3) ELISA Kits anzeigen
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Andere Bezeichnung
Coagulation Factor III (F3 Produkte )
Synonyme
CD142 ELISA Kit, TF ELISA Kit, TFA ELISA Kit, PRO1557 ELISA Kit, PRO2086 ELISA Kit, TFQTL1 ELISA Kit, f3 ELISA Kit, AA409063 ELISA Kit, Cf-3 ELISA Kit, Cf3 ELISA Kit, tf ELISA Kit, zgc:112151 ELISA Kit, coagulation factor III, tissue factor ELISA Kit, transferrin ELISA Kit, coagulation factor IIIa ELISA Kit, coagulation factor III ELISA Kit, tissue factor ELISA Kit, coagulation factor IIIb ELISA Kit, coagulation factor III (thromboplastin, tissue factor) S homeolog ELISA Kit, F3 ELISA Kit, TF ELISA Kit, f3a ELISA Kit, tf ELISA Kit, f3b ELISA Kit, f3.S ELISA Kit
Hintergrund
Gene Names: F3 Cf-3 Cf3 Protein names: Tissue factor (TF) (Coagulation factor III) (CD antigen CD142)
Gen-ID
14066
UniProt
P20352
Pathways
Positive Regulation of Endopeptidase Activity , Smooth Muscle Cell Migration , Platelet-derived growth Factor Receptor Signaling
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