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CFHR5 ELISA Kit

CFHR5 Reaktivität: Human Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2702908
  • Target Alle CFHR5 ELISA Kits anzeigen
    CFHR5 (Complement Factor H-Related 5 (CFHR5))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.41-100 ng/mL
    Untere Nachweisgrenze
    0.41 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human Complement Factor H-related 5 (CFHR5) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human Complement Factor H-related 5 (CFHR5)
    Sensitivität
    0.41 ng/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples400 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Aufbereitung der Proben

    For example, add 2 μL of serum or plasma into a tube with 798 μL 1X Assay Diluent to prepare a 400-fold diluted sample. Mix well.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CFHR5 ELISA Kits anzeigen
    CFHR5 (Complement Factor H-Related 5 (CFHR5))
    Andere Bezeichnung
    CFHR5 (CFHR5 Produkte)
    Synonyme
    CFHL5 ELISA Kit, CFHR5D ELISA Kit, FHR-5 ELISA Kit, FHR5 ELISA Kit, complement factor H related 5 ELISA Kit, CFHR5 ELISA Kit
    Hintergrund
    Gene Names: CFHR5 CFHL5 FHR5
    Protein names: Complement factor H-related protein 5 (FHR-5)
    Gen-ID
    81494
    UniProt
    Q9BXR6
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