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Cadherin 13 ELISA Kit

This anti-Cadherin 13 antibody (ABIN2702905) is a antibody detecting Cadherin 13 in ELISA. Suitable for Human.
Produktnummer ABIN2702905

Kurzübersicht für Cadherin 13 ELISA Kit (ABIN2702905)

Target

Alle Cadherin 13 (CDH13) ELISA Kits anzeigen
Cadherin 13 (CDH13)

Reaktivität

  • 2
  • 2
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

40-10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma, Serum
  • Untere Nachweisgrenze

    40 pg/mL

    Verwendungszweck

    Human Cadherin-13 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

    Analytische Methode

    Quantitative

    Spezifität

    The antibody pair provided in this kit recognizes Human Cadherin-13 (CDH13)

    Sensitivität

    40 pg/mL

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise

    Recommended Dilution for serum and plasma samples50 fold

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target Alle Cadherin 13 (CDH13) ELISA Kits anzeigen

    Cadherin 13 (CDH13)

    Andere Bezeichnung

    CDH-13

    Hintergrund

    Gene Names: CDH13 CDHH
    Protein names: Cadherin-13 (Heart cadherin) (H-cadherin) (P105) (Truncated cadherin) (T-cad) (T-cadherin)

    Gen-ID

    1012

    UniProt

    P55290

    Pathways

    EGFR Signaling Pathway, Cell-Cell Junction Organization
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