BMP7 ELISA Kit

Produktnummer ABIN2702870

Produktdetails BMP7 ELISA Kit

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 4-1.000 pg/mL

Untere Nachweisgrenze: 4 pg/mL

Applikation: ELISA

Verwendungszweck: Mouse BMP-7 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects mouse and human BMP-7. Other species not determined.

Sensitivität: 4 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Andere Bezeichnung: BMP-7 (BMP7 Produkte)

Synonyme: bmp-7 ELISA Kit, bmp7 ELISA Kit, op-1 ELISA Kit, BMP7 ELISA Kit, zgc:153025 ELISA Kit, OP-1 ELISA Kit, OP1 ELISA Kit, BMP-7 ELISA Kit, fb83f09 ELISA Kit, wu:fb83f09 ELISA Kit, bone morphogenetic protein 7 ELISA Kit, bone morphogenetic protein 7, gene 1 L homeolog ELISA Kit, bone morphogenetic protein 7b ELISA Kit, bone morphogenetic protein 7, gene 2 L homeolog ELISA Kit, bone morphogenetic protein 7a ELISA Kit, BMP7 ELISA Kit, bmp7.1.L ELISA Kit, LOC100136282 ELISA Kit, bmp7b ELISA Kit, Bmp7 ELISA Kit, LOC100008826 ELISA Kit, bmp7.2.L ELISA Kit, bmp7a ELISA Kit

Hintergrund: Gene Names: Bmp7 Bmp-7 Op1
Protein names: Bone morphogenetic protein 7 (BMP-7) (Osteogenic protein 1) (OP-1)

Gen-ID: 12162

UniProt: P23359

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance