FITC Annexin V Apoptosis Detection Kit with PI

Details zu Produkt Nr. ABIN2669213, Anbieter: Anmelden zum Anzeigen
Antigen
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin A5 L homeolog
  • ANXA5
  • anxa5b
  • anxa5
  • Anxa5
  • anxa5.L
Reaktivität
Säugetier
19
15
15
14
14
8
7
7
4
2
2
2
2
1
1
1
1
Konjugat
FITC
Applikation
Multiplex Assay (MA)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
Bestandteile FITC Annexin V Apoptosis Detection Kit with PI
Andere Bezeichnung Annexin V (ANXA5 ELISA Kit Abstract)
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Vorsichtsmaßnahmen Caution: Propidium Iodide Solution is toxigenic and mutagenic, handle with care.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store between 2°C and 8°C.
Bilder des Herstellers
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213) FITC Annexin V Apoptosis Detection Kit with PI
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213) FITC Annexin V Apoptosis Detection Kit with PI (Image 2)
Allgemeine Veröffentlichungen Jaishy, Zhang, Chung, Riehle, Soto, Jenkins, Abel, Cowart, Van Eyk, Abel: "Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity." in: Journal of lipid research, Vol. 56, Issue 3, pp. 546-61, 2015 (PubMed).

Wang, Zhao, Yang, Bi, Chi, Tian, Li: "High-dose alcohol induces reactive oxygen species-mediated apoptosis via PKC-β/p66Shc in mouse primary cardiomyocytes." in: Biochemical and biophysical research communications, Vol. 456, Issue 2, pp. 656-61, 2014 (PubMed).

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